Article

Article title STUDY OF THE USE OF INFLUENZA THERMOTAXIS FOR WATER MEDIA EXPRESS MONITORING
Authors S. Zaharov, A. N. Velichko, V. Yu. Vishnevetsky
Section SECTION II. METHODS OF DIAGNOSIS OF THE ECOLOGICAL STATE
Month, Year 08, 2017 @en
Index UDC 534
DOI 10.23683/2311-3103-2017-8-78-88
Abstract The aim of the work is the development of an express method for the control of aquatic envi-ronments toxicity on the basis of test-reaction of the infusorians thermotaxis. The creation of bio-logical monitoring is shown as a regular stage in the development of a civilized society, which, on its historical experience, realized that technological progress can pollute water areas and water necessary for people on land and at sea. The need for this coordination between state power, re-gional, private initiative and scientists" ideas is briefly explained. Important is the ideological transition from toxicology, which protects a human, to protection of ecosystems and the develop-ment of ecotoxicology. The article highlights the inadequacy of several tests for solving environ-mental monitoring problems, since each organism has its own adaptation ranges, and there are many types of toxicity. It is emphasized that the ideas of earlier times are useful for new develop-ments. Attention is drawn to the work of the end of the XIX century, where in vivo experiments were more often, so they studied them in a temperature gradient, in the works of the XX century, a technique was created, in the XXI century the reaction to temperatures was linked to the toxicity of aquatic environments. The urgency of the development of a new microbiotest in vivo from the field of ecotoxicology is substantiated. MicrobiotaTest is based on the test-reaction of thermotaxis of infusorians, the effect of which depends on the toxicity of the aquatic environment. The main con-ditions for the appearance of thermotaxis are revealed. The means for the formation of the thermotaxis of P. caudatum infusorians and the means for recording the process on the basis of video recording and data processing programs have been developed. The explanations of the test reaction formation, the selection of informative parameters, the graph of the impulses and the dependencies of their elements on the concentration of test organisms are given. An experimental device for investigating the effects of inhibitors of cellular respiration and metabolism has been created. For the first time, a method for determining the toxicity of aqueous media based on the test-reaction of infusoria thermotaxis has been proposed, and this technique has been tested on aqueous solutions of model toxicants NaN3 and CuSO4.

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Keywords Biotesting; express biotest; thermotaxis; aqueous media; Paramecium caudatum; bioassay; rapid-bioassay; thermotaxis; aqueous media.
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